Tissue Culture Lab Protocols & Techniques

Collected Cell Culture Protocols (PDF)

Instrument Protocols & Techniques

UV-Vis Use Guidelines (PDF)

Organic Synthesis Techniques & Protocols

There are some really useful resources for organic techniques online, some of which I'll link here:

For TLC Stains, I particularly like the utility of Vanillin- a good recipe and primer can be found at Curly Arrow's blog: TLC Stains Part 1- Vanillin from Curly Arrow

Column Chromatography Basics- JoVE and Welder's Lab at Dartmouth

For NMR Analysis, I can't recommend SpinWorks enough- it's free, open source, and developed by chemists at the University of Manitoba

Working with sintered (fritted) glass, a primer from ILPI

Working under inert atmosphere techniques from ChemistryViews: Part 1, Part 2, Part 3 and Part 4. The same author also has some great tutorials on techniques for crystal growth.

For an excellent collection of tips, tricks, and general protocols, I highly recommend Not Voodoo, a curated collection of organic techniques from the Frontier Lab at University of Rochester, supported by the NSF.

Data Analysis Protocols & Techniques

Determination of Binding Constants from Spectroscopic Titrations (PDF)

Determining Thermodynamic Data from DNA Melting Curves- Van't Hoff Method (PDF)

Protocols & Techniques for Nucleic Acids

Selecting miRNA Targets & Designing Sensors (PDF)

Purification of Synthetic DNA (PDF)

Buffer Selection & Preparation (PDF)

Polyacrylamide Gel Electrophoresis of Oligonucleotides (PDF)